Revista de Obstetricia y Ginecología de Venezuela
Print version ISSN 0048-7732
NIETO-DIONISIO, Karina et al. Comparación entre Testsimplets® y Diff-Quik para la evaluación de la morfología espermática. Rev Obstet Ginecol Venez [online]. 2011, vol.71, n.1, pp. 28-33. ISSN 0048-7732.
Objective: To compare sperm morphology by Testsimplets® and Diff-Quik staining. To compare the efficiency of Staining cellular peroxidase and Testsimplets® to assess leukocytes in semen. Methods: We compared sperm morphology of 30 infertile patients evaluated by Testsimplets® and Diff-Quik staining. Additionally, in patients with more than 106 round cells per ml semen (n=7), we compared the assessment of leukocytes by Staining cellular peroxidase and Testsimplets®. Setting: Fertility clinic UNIFERTES, in Caracas, Venezuela. Results: There was no significant difference in the percentage of morphologically normal sperm, according to Krugers strict criteria, between both stains. We found significant differences in the percentages of the following sperm morphological defects between staining: pyriform head, folded middle piece, irregular and presence of cytoplasmic droplets. There was no significant difference in the assessment of leukocytes in semen by Staining cellular peroxidase and Testsimplets®. Conclusions: Testsimplets® is practical for saving time and consequently, improving the efficiency in the delivery time of results. Nonetheless, it is considerably more expensive than Diff-Quik.
Keywords : Testsimplets®; Diff-Quik; Sperm morphology; Kruger strict criteria; Peroxidase Test; Leukocytospermia.